Glen or Glenda

Bela Lugosi

Scientist

Edward D. Wood Jr.

Glen / Glenda (as Daniel Davis)

Lyle Talbot

Inspector Warren

Timothy Farrell

Dr. Alton / Narrator

Dolores Fuller

Barbara

'Tommy' Haynes

Alan / Anne

Charlie Crafts

Johnny (as Charles Crafts)

Conrad Brooks

Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Henry Bederski

Man with Hat and Receding Hairline (uncredited)

Carol Daugherty

Woman in Nightmare (uncredited)

'Captain' DeZita

The Devil / Glen's Father (uncredited)

Helen Miles

(uncredited)

Shirley Speril

Miss Stevens (uncredited)

Amzie Strickland

Minor Role (uncredited)

Harry Thomas

Man in Nightmare (uncredited)

William C. Thompson

Judge (uncredited)

'Mr. Walter' Hadjwiecyz

Patrick / Patricia (uncredited)

George Weiss

Man at Transvestite's Suicide (uncredited)